Dewey Edition23
Reviews'This book is an essential read for embryologists at all stages of their careers … This book will prompt reflection on current laboratory practice and has sparked lively and thought-provoking discussions with my colleagues. No embryologist should be without it!' Lucy Richardson, The Bulletin, "This book is an essential read for embryologists at all stages of their careers ... This book will prompt reflection on current laboratory practice and has sparked lively and thought-provoking discussions with my colleagues. No embryologist should be without it!" Lucy Richardson, The Bulletin
Table Of ContentPreface; Acknowledgements; 1. Introduction; 2. Quality control, certification and accreditation; 3. The IVF culture system; 4. Osmometry; 5. pH regulation in gamete and embryo culture systems; 6. Temperature control in the IVF laboratory; 7. The effect of light on embryos and embryo culture; 8. Gases, air quality and volatile organic carbons (VOCs); 9. Infections in IVF culture; 10. Troubleshooting a cryopreservation system; 11. Troubleshooting ICSI procedures; 12. Managing risk in an IVF lab; Index.
SynopsisMaintaining consistent and reliably high success rates is a daily challenge for every IVF laboratory. This step-by-step guide is an essential aid in navigating the complex maze of physical, chemical, biological, and logistic parameters that underpin successful gamete and embryo culture: temperature, pH, osmolality, gas supplies, air quality, light exposure, infections, managing supplies, personnel, as well as overall quality control. Numerous real-life troubleshooting case reports are presented, identifying all aspects necessary for troubleshooting. Process maps and flow charts accompanying each chapter offer a logical and systematic approach to problem solving in the laboratory. This is an essential resource for scientists in assisted reproductive technology and specialists in reproductive biology and medicine, helping IVF clinics to achieve the dream of every infertile couple: the birth of a healthy child., This step-by-step guide navigates the maze of physical, chemical, biological and logistic parameters underpinning successful gamete and embryo culture. All aspects necessary for troubleshooting are identified, offering a systematic approach to problem-solving in the laboratory. An essential resource for all scientists in assisted reproductive technology and specialists in reproductive biology and medicine.